Deutsch. University Logo. Home; Search; Browse; Submit; Sitemap. Schlie?en. Development of tools for inducible gene expression in choroplasts. Masoumeh Emadpour. Export metadata. BibTeX; RIS; XML. Additional Services. Share in Twitter Search Google Scholar Statistics. Metadaten. Author: Masoumeh Emadpour. Document Type: Doctoral Thesis. Language: English. Year of Completion: 2014. Granting Institution: Universit?t Potsdam. Release Date: 2015/04/09. Pagenumber: viii. Organizational units: Mathematisch-Naturwissenschaftliche Fakult?t / Institut f?r Biochemie und Biologie. Dewey Decimal Classification: 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie. OPUS4 Logo KOBV Logo OAI Logo DINI Ze

In temperate regions, subsequent to apple, pear is one of the important crops. Traditional pear breeding involves hybridization between and within different species. However, due to high levels of heterozygosity, polygenic characters and long juvenile stage, traditional breeding is considered to be very time consuming and expensive. Therefore, using modern biotechnology methods such as genetic engineering has become popular. In the present investigation gene transformation of two pear cultivars (" Harrow Delight" and" Bartlett") using agrobacterium were studied. In gene transformation by agrobacterium, EHA101 and LBA4404 strains of agrobacterium harboring pBI121-GUS and kanamycin resistant nptII gene were used. The following parameters: typ

Genetic engineering technique could be used to increase rape seed yield and also increase the disease and pest resistance in this valuable crop. Genetic engineering to microspores and production of haploid cultures is one of the new techniques which is being used recently. The main technique for haploid production in Brasica is microspore culture, which the technique has been registered for many species and important commercial genotypes. The first stage in gene transformation to microspore is identification of the developmental stages of the microspore growth and also the factors affecting its isolation and embryogenesis. In the present investigation the factors affecting gene transformation of rape seed microspores using the gus gene were

Internal and external accumulation of ethylene is considered as one of the most significant causes of post-harvest losses in cut leafy vegetables including lettuce and cabbage. Ethylene is an important factor in the ripening of fruits and is naturally produced in most plant tissues. Its production and accumulation during the storage can cause serious damage to the stored vegetables such as lettuce and cabbage. It plays a significant role in shelf life and can cause a marked increase in respiration rates and enhanced senescence. In vegetables and cut flowers, accelerated aging and the initiation of ripening can occur following exposure to ethylene at concentrations as low as 0.1 ml/l. As senescence begins, spoilage due to indigenous bacteria

Powdery mildew disease causes yield and quality reduction in plants of Rosaceae family every year. In order to create resistant rose plants to this disease, plant regeneration was first optimized. The best regeneration was obtained using leaf explants cultured in medium containing 2, 4-D and NAA. After optimizing tissue culture stage, changing the construct containing Kitinase gene (chi) was done by selecting suitable vector and its transfer to E. coli cells and to Agrobacterium tumofacience. This was achieved by cloning Kitinase protein encoding gene of one kb length into pGEM-7Zf (+) transporter. This plasmid was transferred to isolate XLI-Blue of E. coli. Conjugated Kitinase gene was subsequently cut using two restriction enzymes and the

Traditional pear breeding involves hybridization between and within different species. However, due to high levels of heterozygosity, polygenic characters and long juvenile stage, traditional breeding is very time consuming and expensive. Therefore, using modern biotechnology methods such as genetic engineering and mutation has become popular. In this regard effective and repeatable in vitro regeneration protocols are the prerequisites for in vitro breeding. The aim of the present project was to develop an effective direct and indirect regeneration method for three commercially important pear cultivars (Dare Gazy, Harrow Delight and Bartlett). For direct regeneration; culture media (Nitsch) containing different concentrations of TDZ (0, 2.5

This research was carried out in Gene Transfer and Tissue Culture Laboratory of Agricultural Biotechnology Research Institute of Iran (ABRII) in 2008. Fire blight caused by the bacterium, Erwinia amylovra (Burrill), reduces the yield and quality of Pomoideae growing areas annually. The aim of this study was isolation and cloning of the gene encoding harpin protein (hrpN) from E. amylovra strain 273 and construction of pBI-hrpN recombinant plasmid in order to widespread fire blight resistance strategy development based on expression of defense response of pear (Pyrus communis). Specific primers (forward and reverse) were designed for amplifying the gene (hrpN) using complete sequence of genomic DNA of E. amylovora strain 273. Then, to carry